The Role of Biological Supplements
in Establishing a Healthy Aquatic Ecosystem.


A microbe is any living thing that spends its life at a size visible only with a microscope. It is too tiny to be seen with the naked eye. Microbes are the oldest form of life on Earth. Some types have existed since the earth was first created. They may live as individuals or cluster together in communities. Microbes live in the water you drink, the food you eat, and the air you breathe. Right now, billions of microbes are swimming in your belly and mouth, and crawling on your skin. Don't worry, over 95% of microbes are good for you and most of the rest are harmless. Microbes include bacteria, viruses, fungi, algae, and protozoa.

In this chapter, we will discuss how the addition of specifically selected bacteria can stabilize and optimize many of the natural processes in your pond's ecosystem. In converting from ammonia to nitrate there are two bacterial species involved. Nitrosomonas sp. bacteria which oxidize ammonia to nitrite, while Nitrobacter bacteria convert nitrite to nitrate, with both species utilizing the energy released by the reactions. This process involves a complex series of reactions that can be summarized in chemist shorthand as this:

For Nitrosomonas: 55NH 4+ + 76 O 2 + 109HCO 3 C 5 H 7 O 2 N + 54NO 2 + 57H 2 O + 104H 2 CO 3

For Nitrobacter: 400NO 2 + NH 4 + + 4H 2 CO 3 + HCO 3 + 195 O 2 C 5 H 7 O 2 N + 3H 2 O + 400 NO 3

What these reactions show is that approximately 4.3 mg of O 2 ( oxygen) are consumed for every mg of ammonia-nitrogen oxidized to nitrate-nitrogen and 8.64 mg of alkalinity in the form of HCO 3 are consumed per mg of ammonia-nitrogen oxidized.

This is a substantial amount of alkalinity reduction and will, over a period of time, dramatically change the character of the pond water, affecting both hardness and pH stability. It is also a mild acidifying process, producing a gradual build up of nitric acid. It should also be noted that the process does not remove any nitrogen from the system it simply changes it from one form to another. Nitrification uses substantial amounts of oxygen and carbonate, thus reducing water hardness and lowering pH.

So what about the nitrate? Although nitrate does not represent a direct health threat to most fish, high levels are still undesirable. Nitrate is the one of the basic food sources for aquatic weeds and algae so we can see that increasing the nitrate in the system could create more problems by stimulation growth. There is one final phase of this process necessary to remove excess nitrogen from the ecosystem. Denitrification and dissimilation are parts of another natural process that converts nitrate to atmospheric nitrogen gas. This process only occurs in the absence of oxygen. This is why a small amount of bottom sediment is a necessary part of a healthy ecosystem. The sediment in the benthic layer on the pond bottom is a low oxygen environment where these reactions can occur. The first stage is dissimilatory nitrate reduction, which reverses the nitrification process and converts nitrate (NO 3 ) back to nitrite (NO 2 ). The second stage of denitrification converts nitrite to nitric oxide, nitrous oxide and finally nitrogen gas All of these last three products are gases that can be released into the atmosphere.

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The bacteria used in these formulations are derived  generally from natural environments and are not genetically engineered. Useful bacterial strains are grown and blended for application to specific organic problems and commercial uses and typically contain a number of species that have been selected and combined to degrade specific organic compounds or waste products. The strains of bacteria in the blend work together - each producing the enzymes necessary to degrade specific components in the waste and their resulting intermediates until reaching non-harmful compounds such as water and carbon dioxide.
By adding supplemental bacteria to the pond water, a natural cleansing process is established using biologically active bacteria cultures, enzymes, activators and accelerators developed specifically for ponds. The microbes populate the entire system, multiply and produce large quantities of digestive enzymes on an ongoing basis.

The  bacterial cultures will help break down ammonia to form nitrates and nitrites which are then reduced to nitrogen gas. Similarly, other waste materials in the pond, like waterfowl excrement and landscape debris, are broken down in the system into non-hazardous materials. Unless treated with bacteria, these materials form oxygen-depleting bottom sludge. The break down process gives the bacteria the nutrients they require to grow and reproduce.

The bacteria utilize dissolved oxygen in their digestive processes. However, by removing the undesirable organic materials noted above and reducing BOD in the aquatic system, ultimately, more oxygen will be available in the system. The use of bacteria will not adversely affect a well-aerated pond.

The use of supplemental bacteria is not a one-time quick fix for poorly circulated or eutrophic ponds. Rather it is a preventative maintenance tool that requires bacterial additions at regularly spaced intervals.  Using microbial treatments in your lake on an ongoing basis will assure that your ponds' ecosystem stays healthy and under control. These treatments will help control nutrient levels, reduce sediment, stabilize pH, alkalinity and oxygen levels and bring your pond into optimum condition, when used in conjunction with other components of a balanced treatment program, including proper circulation and the selective use of algaecides and herbicides needed to control fresh aquatic growths.

One word of caution, if your pond has poor circulation and low oxygen content, this process can occur throughout the pond, resulting in excessive nitrite being fed back into the system, causing mysterious fish kills. This is possible during very warm weather when oxygen demand is at its highest, but dissolved oxygen levels at their lowest. During the night when oxygen levels fall to their lowest because of algal respiration, it is possible for oxygen levels to fall below the critical level in a poorly aerated system that is loaded with decomposing organic matter. The situation will be reversed at sun-up when photosynthesis restarts and oxygen is released back into the water, leaving the pond-keeper confused while trying to explain the dead fish he finds each morning. The remedy for this situation is to have adequate circulation or aeration installed in the pond. Circulation is critical to good pond maintenance for a variety of reasons. We will discuss this in the next chapter.

Bioaugmentation is a completely natural and ecologically harmless method of maintaining a proper pond. Typically, small and medium sized ponds have difficulty controlling excess nitrates and ammonia in their confined systems. The buildup of nitrates and ammonia create toxic environments that result in fish kills and eutrophication of the system. These problems can be controlled by the addition of supplemental bacteria.

This process will occur in and near the bottom sediment of the pond called the benthic layer. Oxygen levels are very low in this zone because of the high BOD (biological oxygen demand) within this sediment. Heterotrophic bacteria that derive their energy from oxidation and consumption or absorption of other organisms, when confronted with a low oxygen condition, will switch to this nitrate reduction, thus removing nitrogen from the system as escaping gasses. These same bacteria are responsible for the reduction of the organic sediment on the lake bottom, keeping this build-up from becoming a serious problem. Ponds that are maintained in this way will develop a natural layer of sediment consisting of mostly inorganic material that the microbes do not break down. This sediment layer is a necessary part of the bioremediation process. The practices of draining smaller ponds and removing the sediment or vacuuming the sediment out can actually disrupt the established ecosystem.
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